Coding

Part:BBa_K3629005

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-11)
Revision as of 04:23, 12 October 2020 by Sravyakakumanu (Talk | contribs)


Modified Penicillium funiculosum CBHI with 6X His tag

Optimized cellobiohydrolase I coding sequence from Penicillium funiculosum with 6x HIS tag.

Usage and Biology

Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 564
    Illegal BamHI site found at 1048
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
Parameters
None