![](https://parts.igem.org/images/partbypart/icon_terminator.png)
Terminator
Part:BBa_K3629004
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-11)
Revision as of 04:05, 12 October 2020 by Sravyakakumanu (Talk | contribs)
Yarrowia lipolytica XPR2 terminator
TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from Y. lipolytica (BBa_K1592000)
Usage and Biology
Design
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 271
Illegal EcoRI site found at 265 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 265
Illegal SpeI site found at 272
Illegal PstI site found at 286
Illegal NotI site found at 7
Illegal NotI site found at 279 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 265 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 272
Illegal EcoRI site found at 265 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 265
Illegal XbaI site found at 16
Illegal SpeI site found at 272
Illegal PstI site found at 286 - 1000COMPATIBLE WITH RFC[1000]
References
[edit]
Categories
Parameters
None |