Coding

Part:BBa_K3629019

Designed by: Arshia Mostoufi   Group: iGEM20_Calgary   (2020-10-09)
Revision as of 03:51, 12 October 2020 by Sravyakakumanu (Talk | contribs)


Yarrowia lipolytica LEU4 gene

2-isopropylmalate (LEU4) gene from Yarrowia lipolytica .

Usage and Biology

The YALI0B07447g is the gene for Yarrowia lipolytica's 2-isopropylmalate synthase which is a LEU4 or

2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme specially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 728
    Illegal BamHI site found at 293
    Illegal XhoI site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 799
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 544


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Categories
Parameters
None