Protein_Domain

Part:BBa_K3629003

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-10)
Revision as of 23:32, 11 October 2020 by Sravyakakumanu (Talk | contribs)


Yarrowia lipolytica EXP Promoter

The part contains wild-type TEF1 promoter from Yarrowia lipolytica and the first 42nts (14 codons) of the Lip2 signal peptide from Y. lipolytica (BBa_K1592000).


Usage and Biology

Design

Upon digestion with BbsI and this part can be assembled into a destination vector (containing (BBa_K3629012) with a coding sequence that contains the last 57nts (19 codons) of the Lip2 signal peptide at the 5' end by using Gibson Assembly. In our part collection, this part can be assembled with ________ into the BBa_K3629012 destination vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1025
    Illegal SpeI site found at 602
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 663
    Illegal SpeI site found at 602
    Illegal SpeI site found at 1026
    Illegal PstI site found at 1040
    Illegal NotI site found at 7
    Illegal NotI site found at 1033
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1026
    Illegal SpeI site found at 602
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 602
    Illegal SpeI site found at 1026
    Illegal PstI site found at 1040
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
Parameters
None