Coding
Cr-atzA B3

Part:BBa_K3373001:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-11)
Revision as of 23:04, 11 October 2020 by Registry (Talk | contribs)

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atzA P. sp. ADP codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 825
    Illegal PstI site found at 1389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 825
    Illegal PstI site found at 1389
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 825
    Illegal PstI site found at 1389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 825
    Illegal PstI site found at 1389
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 653
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends.

The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.


Source

Pseudomonas sp. ADP strain.

References