Composite
Part:BBa_K3394004
Designed by: Talia Shaler Group: iGEM20_BGU-Israel (2020-10-11)
Cellulolytic complex [1]: His-Endo5a-GS-CBD
Since cellulose cannot cross the membrane, we engineered E. coli to express cellulolytic complex on its surface through AIDA-1 auto-display system.
Our cellulolytic complex includes cellulase endo5a linked to CBD (cellulose binding domain) through a GS-linker and a 6-histidine tag (His-tag) at the N-terminus.
This complex was designed to be experssed on E.coli's surface through AIDA-1 auto-display system, since cellulose cannot cross the membrane.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314
Illegal BamHI site found at 28 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314
Illegal AgeI site found at 1289 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |