Composite
Part:BBa_K3610050
Designed by: Jonas Sebastian Trottmann Group: iGEM20_UZurich (2020-10-10)
CORE ectodomain / mCherry C-terminal for C. reinhardtii
This part includes the ectodomain of the plant pattern recognition receptor CORE fused to the N-terminal domain of the split-mCherry protein. The sequence for the receptor and the mCherry protein have been codon optimized for expression in C. reinhardtii. To ensure localization at the membrane, this part further contains the sequence for the signal peptide SP7 from C. reinhardtii and the self-cleaving protein from the foot and mouth virus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 148
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1071
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1917
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Categories
Parameters
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