Composite
Part:BBa_K3610042:Design
Designed by: Jonas Sebastian Trottmann Group: iGEM20_UZurich (2020-10-10)
EFR ectodomain / mCherry C-terminal for C. reinhardtii
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 235
Illegal NheI site found at 1165
Illegal NheI site found at 2083 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 148
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1751
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
All codon optimization was done with SnapGene
Source
BBa_K2136017, BBa_K3610012, BBa_K3610008, BBa_K416001, BBa_K1150016, BBa_K3610019