RNA

Part:BBa_K3380501

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-09)
Revision as of 09:50, 10 October 2020 by Alexandru popov (Talk | contribs)


Broccoli fluorescent RNA aptamer construct under T7 RNA polymerase promoter (BBa_z0251)

The Edinburgh iGEM team 2020 designed a construct comprising a fluorescent RNA aptamer (Broccoli BBa_K3380153) flanked by a tRNA scaffold (F30 BBa_K3380101 and BBa_K3380102) under a strong class III T7 RNA polymerase promoter (BBa_z0251). It was designed to test the fluorescence intensity of the iSpinach aptamer flanked by F30 tRNA scaffold when binding to the DFHBI-1T fluorophore. The construct is capable of exhibiting fluorescence being a transcription only construct. The T7 RNA polymerase is capable of synthesizing transcripts via run-off transcription in the absence of a terminator. Figure 1 illustrates the detailed design of the construct. [File:BBa K3380501.png]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


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Parameters
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