Reporter

Part:BBa_K3380150:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-08)
Revision as of 09:18, 10 October 2020 by Alexandru popov (Talk | contribs) (→‎Source)


iSpinach fluorescent RNA aptamer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The iSpinach RNA aptamer was flanked by the F30 tRNA scaffold BBa_K3380101 and BBa_K3380102 for increased fluorescence and stability.

Source

iSpinach is an artificial RNA aptamer that was obtained using the SELEXapproach. It is based on the Spinach fluorescent RNA aptamer.

References

Autour, A., Westhof, E. and Ryckelynck, M., 2016. iSpinach: a fluorogenic RNA aptamer optimized for in vitro applications. Nucleic acids research, 44(6), pp.2491-2500.