RNA

Part:BBa_K3380101:Design

Designed by: Ying Yang, Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-07)
Revision as of 08:58, 10 October 2020 by Alexandru popov (Talk | contribs) (→‎Design Notes)


F30 Upstream RNA aptamer scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ordered the synthesized sequence. Originally ordered sequence: forward (5’ TCCCttgccatgtgtatgtggg 3’) and (5’AGTAcccacatacacatggcaa 3’) with TCCC and AGTA respectively being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters). The forward and reverse sequences were phosphorylated, annealed and ligated to other parts using T4 DNA ligase.

Source

Reengineered from a natural three-way junction structure.


References

Filonov, G.S., Kam, C.W., Song, W. and Jaffrey, S.R., 2015. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chemistry & biology, 22(5), pp.649-660.