RNA
Part:BBa_K3380101:Design
Designed by: Ying Yang, Alexandru Popov Group: iGEM20_Edinburgh (2020-10-07)
Revision as of 08:54, 10 October 2020 by Alexandru popov (Talk | contribs)
F30 Upstream RNA aptamer scaffold
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We ordered the synthesized sequence. Originally ordered sequence: 5’ TCCCttgccatgtgtatgtggg 3’ with TCCC being a linker, to facilitate further manipulations (swapping of the part or attaching to different promoters).
Source
Reengineered from a natural three-way junction structure.
References
Filonov, G.S., Kam, C.W., Song, W. and Jaffrey, S.R., 2015. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chemistry & biology, 22(5), pp.649-660.