![](https://parts.igem.org/images/partbypart/icon_rna.png)
RNA
Part:BBa_K3380101:Design
Designed by: Ying Yang, Alexandru Popov Group: iGEM20_Edinburgh (2020-10-07)
Revision as of 08:27, 10 October 2020 by Alexandru popov (Talk | contribs)
F30 Upstream RNA aptamer scaffold
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Commonly used RNA scaffolds exhibit unexpected instability and cleavage in bacterial and mammalian cells, thus the negative points must be controlled. The reengineered F30 showed it can enhance the brightness of mammalian cells expressing cassette-tagged RNAs.
Source
Reengineered from a natural three-way junction structure.
References
Filonov, G.S., Kam, C.W., Song, W. and Jaffrey, S.R., 2015. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chemistry & biology, 22(5), pp.649-660.