RNA

Part:BBa_K3380101:Design

Designed by: Ying Yang, Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-07)
Revision as of 08:27, 10 October 2020 by Alexandru popov (Talk | contribs)


F30 Upstream RNA aptamer scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Commonly used RNA scaffolds exhibit unexpected instability and cleavage in bacterial and mammalian cells, thus the negative points must be controlled. The reengineered F30 showed it can enhance the brightness of mammalian cells expressing cassette-tagged RNAs.


Source

Reengineered from a natural three-way junction structure.


References

Filonov, G.S., Kam, C.W., Song, W. and Jaffrey, S.R., 2015. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chemistry & biology, 22(5), pp.649-660.