RNA
Part:BBa_K3380101
Designed by: Ying Yang, Alexandru Popov Group: iGEM20_Edinburgh (2020-10-07)
Revision as of 08:13, 10 October 2020 by Alexandru popov (Talk | contribs)
F30 Upstream RNA aptamer scaffold
Short RNA sequence that is transcribed into a tRNA scaffold, it can help improve the fluorescence of RNA aptamers such as iSpinach (BBa_K3380150)
RNA aptamers can be expressed in cells to influence and image cellular processes. Aptamer folding is maintained by inserting the aptamers into highly structured tRNA scaffolds. The F30 tRNA scaffold, besides facilitating the fluorescent RNA aptamer folding, helps protect it from being degraded by the RNAses.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |