Regulatory

Part:BBa_K3504004

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-09-19)
Revision as of 18:31, 9 October 2020 by Ahmed Wael (Talk | contribs) (→‎Improvements)


ِِAlphavirus Subgenomic Promoter 30

Part Description

Alpha-virus replicon Subgenomic constitutive promoter. And a subgenomic promoter is a promoter added to a virus for a specific heterologous gene resulting in the formation of mRNA for that gene alone.

Usage

Characterization

Improvements

We Optimized our SGP and choose between multiple sequences to achieve the best sequence with highest stability and lowest energy

Figure 1. Optimized SGP RNA fold.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None