Coding
NSP4
Part:BBa_K3504003
Designed by: Ahmed Wael Group: iGEM20_AFCM-Egypt (2020-09-19)
nSP4-RNA-directed RNA Polymerase-Eastern equine encephalitis virus
Part Description
Nsp4 is one of four non structural proteins that together forms the main complex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is produced in excess of the viral genome. Which allows the virus to self-replicate into millions of copies of the virus.
Usage
Characterization
Improvements
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 948
Illegal NheI site found at 1765 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 19
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |