Composite

Part:BBa_K3593013

Designed by: ZiJiao Wang   Group: iGEM20_GreatBay_SCIE   (2020-10-09)
Revision as of 14:01, 9 October 2020 by Registry (Talk | contribs)

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pelB-scFv(anti alpha-amatinin)-6*His

This part express a soluble form of scFv(anti α-amatinin) in BL21(DE3)(E.coli). The transcription codons are optimized to obtain the best expression in E.coli. pelB tag is a signal peptide link in the N-term of scFv that guide this protein into the oxidizing periplasm that helps the disulfide bond in scFv to form. The 6*His tag in the C-term of scFv helps to purify protein by IMAC and provides epitope for secondary antibody to recognize.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 164
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None