Coding
Cry7Ca1

Part:BBa_K3407001:Design

Designed by: Alicia Rodriguez Molina   Group: iGEM20_TUDelft   (2020-10-08)
Revision as of 11:44, 9 October 2020 by Alicia RM (Talk | contribs)


Cry7Ca1 toxin from Bacillus thuringiensis strain BTH-13


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 634
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1334
    Illegal XhoI site found at 313
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa (Figure 2). The Cry7Ca1 toxin (activated Cry7Ca1 protein) crystal structure has been published and can be found in the Protein Data Bank under the accession number 5ZI1 [1] Ordered List

  1. Jing, X., Yuan, Y., Wu, Y., Wu, D., Gong, P., & Gao, M. (2019). Crystal structure of Bacillus thuringiensis Cry7Ca1 toxin active against Locusta migratoria manilensis. Protein science : a publication of the Protein Society, 28(3), 609–619.