Reporter

Part:BBa_K3380153

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-08)
Revision as of 09:08, 9 October 2020 by Alexandru popov (Talk | contribs) (→‎Characterization)


Broccoli fluorescent RNA aptamer

Broccoli is a fluorescent RNA aptamer comprising a short ribonucleotide sequence that exhibits fluorescence upon binding to a specific fluorophore.

Characterization

The Broccoli aptamer was designed and described by Filonov et al. (2014). The Broccoli secondary structure folding prediction as simulated by [http://www.nupack.org// NUPACK] is shown below.

Broccoli folding

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None