Composite

Part:BBa_K3408007

Designed by: Chen Peng   Group: iGEM20_NAU-CHINA   (2020-10-06)
Revision as of 12:04, 8 October 2020 by Tequilasunrise (Talk | contribs)


Pnar-B0034-phy(ycD)-B0015

Used Pnar(BBa_K3408000), RBS(BBa_B0034), optimized CⅠ(BBa_K3408004), terminator(BBa_B0015), PCⅠ(BBa_R0051), RBS(BBa_B0034) and GFP(BBa_E0040) to express GFP when PCⅠ was activated. When our Bacillus subtilis was in an aerobic environment, Pnar was suppressed without FNR and no CⅠ protein could be expressed so that PCⅠ could be activated and we could see GFP expressed. But when our Bacillus subtilis was in an anerobic environment, CⅠ protein could be expressed to suppress PCⅠ and we couldn't see GFP anymore.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 454
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None