Part:BBa_K3380102:Design
F30 Downstream RNA aptamer scaffold
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The F30 downstream RNA aptamer scaffold needs to be placed after the RNA aptamer sequence followed by the 4bp overhang. It should be used in conjunction with the F30 Upstream RNA aptamer scaffold (BBa_K3380101) for proper functioning.
The short length of the F30 downstream RNA aptamer scaffold, allows its synthesis rather than cloning it using plasmids.
Source
The F30 scaffold was engineered by Filonov et al. in 2015. It is based on the Φ29 RNA three-way junction motif studied by Shu et al. in 2014.
References
Filonov, G.S., Kam, C.W., Song, W. and Jaffrey, S.R., 2015. In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chemistry & biology, 22(5), pp.649-660.
Shu, D., Khisamutdinov, E.F., Zhang, L. and Guo, P., 2014. Programmable folding of fusion RNA in vivo and in vitro driven by pRNA 3WJ motif of phi29 DNA packaging motor. Nucleic acids research, 42(2), pp.e10-e10.