RNA

Part:BBa_K3380102:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-07)
Revision as of 10:06, 8 October 2020 by Alexandru popov (Talk | contribs) (→‎Design Notes)


F30 Downstream RNA aptamer scaffold


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The F30 downstream RNA aptamer scaffold needs to be placed after the RNA aptamer sequence followed by the 4bp overhang. It should be used in conjunction with the F30 Upstream RNA aptamer scaffold (BBa_K3380101) for proper functioning.

Source

The F30 scaffold was engineered by Filonov et al. in 2015. It is based on the Φ29 RNA three-way junction motif studied by Shu et al. in 2014.

References