T7

Part:BBa_K3398000

Designed by: MingXiao Wei   Group: iGEM20_ZJU-China   (2020-10-08)
Revision as of 07:00, 8 October 2020 by Registry (Talk | contribs)

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Enhanced T7 promoter 1

This part is a T7 promoter derived from BBa_I712074 with some point mutation according to previous research [1], and as a higher expression efficiency relative to BBa_I712074 by the fluorescence intensity when expressing EGFP. To express BioBricks under the control of this part, a T7 polymerase gene is needed in the chassis.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None