Composite

Part:BBa_K3349010:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-28)
Revision as of 15:23, 7 October 2020 by Kristiturton (Talk | contribs) (→‎References)

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Pectin Lyase PelB-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 333
    Illegal BamHI site found at 1218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was codon optimized for use in E. coli. The his tag was placed on the C-terminus of the GFP reporter.


Source

The pectase sequence was taken from the Uniprot server. The sequence ID from NCBI is WP_123064018.1. The GFP sequence was taken from uniprot as well (P42212).

References

1. Keggi, C and Doran-Peterson, J. (2020) The homogalacturonan deconstruction system of Paenibacillus amylolyticus 27C64 requires no extracellular Pectin Methylesterase and has significant industrial potential. Applied and environmental microbiology. 86, e02275-19.