![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3408008
Designed by: Yunong Li Group: iGEM20_NAU-CHINA (2020-10-07)
Pnar-B0034-CⅠ-B0015-PCⅠ-B0034-GFP-B0015
We employed Promoter Pnar, CⅠ protein, toehold switch comprising of trigger RNA, and Switch RNA to precisely regulate the expression of suicide protein mazF. As a result, when the engineered bacteria reaches the outside aerobic environment, CⅠ protein ceased to be expressed. Thus Switch RNA is expressed, along with former accumulated Trigger RNA, Toehold switch is triggered. Then our suicide protein mazF is expressed, thus leading to the suicide of our engineered bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1709
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Categories
Parameters
None |