Composite

Part:BBa_K3408008

Designed by: Yunong Li   Group: iGEM20_NAU-CHINA   (2020-10-07)
Revision as of 14:42, 7 October 2020 by Registry (Talk | contribs)

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Pnar-B0034-CⅠ-B0015-PCⅠ-B0034-GFP-B0015

We employed Promoter Pnar, CⅠ protein, toehold switch comprising of trigger RNA, and Switch RNA to precisely regulate the expression of suicide protein mazF. As a result, when the engineered bacteria reaches the outside aerobic environment, CⅠ protein ceased to be expressed. Thus Switch RNA is expressed, along with former accumulated Trigger RNA, Toehold switch is triggered. Then our suicide protein mazF is expressed, thus leading to the suicide of our engineered bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1709


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