Composite

Part:BBa_K3519012

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)
Revision as of 13:56, 7 October 2020 by Registry (Talk | contribs)

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araC-araBAD promoter-DNASEI

This part contains the DNASEI gene downstream to the arabinose inducible promoter. The gene is the bovine pancreatic DNaseI isolated from Bos taurus. This is a tightly regulated 'kill switch'. The gene is expressed on the addition of arabinose to the media. Excess glucose in the media represses the expression of the endonuclease thus preventing cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
    Illegal NheI site found at 1066
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1388
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1223
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1205


[edit]
Categories
Parameters
None