Coding

Part:BBa_K3408004

Designed by: Chen Peng   Group: iGEM20_NAU-CHINA   (2020-10-03)
Revision as of 17:02, 6 October 2020 by NJAU-Chappie (Talk | contribs)


Optimized CⅠ

It is a protein which could suppress PC1 so that our switch couldn't be transcribed without oxygen. It is also under the control of Pnar.

We have optimized the C1 protein on ThermoFisher SCIENTIFIC(https://www.thermofisher.com/cn/zh/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis/geneoptimizer.html) to use it better in our engineered bacteria.

T--NAU-CHINA--basic-parts3.png T--NAU-CHINA--basic-parts4.png

The histograms show the percentage of sequence codons which fall into a certain quality class. The quality value of the most frequently used codon for a given amino acid in the desired expression system is set to 100, the remaining codons are scaled accordingly (see also Sharp, P.M., Li, W.H., Nucleic Acids Res. 15 (3),1987).

T--NAU-CHINA--basic-parts5.png T--NAU-CHINA--basic-parts6.png

The plots show the quality of the used codon at the indicated codon position.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None