Coding

Part:BBa_K3376008

Designed by: Li, Sheng-Fong   Group: iGEM20_Mingdao   (2020-10-05)
Revision as of 16:54, 6 October 2020 by Wilson-Li (Talk | contribs)


Streptococcus sanguinis pyruvate oxidase (spxB)

https://www.uniprot.org/uniprot/A3CKY8

The DNA sequence was optimized for S. mutans UA159, but the function also works well in E. coli as demonstrated in our work.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 658
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 577
    Illegal SapI site found at 1255
    Illegal SapI.rc site found at 1711


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Categories
Parameters
None