Composite

Part:BBa_K3408007

Designed by: Chen Peng   Group: iGEM20_NAU-CHINA   (2020-10-06)
Revision as of 11:18, 6 October 2020 by Registry (Talk | contribs)

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Pnar-B0034-phy(ycD)-B0015

Used PliaG(BBa_K823000), RBS(BBa_B0034), lacI(BBa_C0012), terminator(BBa_B0015), Pgrac(BBa_K1628202), mazF(BBa_K1096002) to avoid expressing mazF when IPTG was added.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 454
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None