Coding

Part:BBa_K3570012

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-03)
Revision as of 07:22, 6 October 2020 by Antonmykhailiuk (Talk | contribs)


Geraniol synthase from Catharanthus roseus

Catharanthus roseus produces monoterpene indole alkaloids (MIAs). Geraniol synthase catalyzes a key step in the generation of geraniol from geranyl diphosphate (GPP) in the monoterpenoid branch of the MIA pathway.

The sequence of the geraniol synthase from Catharanthus roseus (CrGES) has been taken from Genbank (JN882024.1) then truncated to the 43 amino acid in order to increase its activity (Jiang et al.).

References: - GenBank ID: JN882024.1

- Jiang, G.-Z., Yao, M.-D., Wang, Y., Zhou, L., Song, T.-Q., Liu, H., Xiao, W.-H., & Yuan, Y.-J. (2017). Manipulation of GES and ERG20 for geraniol overproduction in Saccharomyces cerevisiae. Metabolic Engineering, 41, 57–66. https://doi.org/10.1016/j.ymben.2017.03.005 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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