Plasmid
pBSEP1
Part:BBa_K090401
Designed by: Daniel Goodman Group: iGEM08_Cambridge (2008-10-27)
Revision as of 03:03, 28 October 2008 by Dbgoodman (Talk | contribs) (New page: This is the BioBrick-compatible backbone for pBSEP1, containing two origins of replication, one for gram-positive, and another for gram-negative bacteria. It has two selectable resistance ...)
This is the BioBrick-compatible backbone for pBSEP1, containing two origins of replication, one for gram-positive, and another for gram-negative bacteria. It has two selectable resistance markers, one for Chloramphenicol (5µg/ml) in Bacillus subtiis, and one for Ampicillin (100 µg/ml) in E. coli.
This vector can be used to construct large BioBrick parts using traditional gram-negative-based transformation and minipreps, and then also be used to hold the completed part for expression in a gram-positive chassis.
Note that unlike the image, this vector is empty and does not contain any other BioBricks between the cut sites.
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