Composite

Part:BBa_K3610036

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-31)
Revision as of 13:22, 5 October 2020 by Jtrott (Talk | contribs)


BAK1 ectodomain / mCherry N-terminal - for C. reinhardtii

This part is the cell surface receptor BAK1 from A. thaliana fused with the N-terminal sequence of a split-mCherry protein. The two protein coding sequences are fused together with a 15 amino acid linker, coding for glycin and serine. As a promoter a truncated version of the ADH1 version was used as it confers lower expression levels. To mediate termination of transcription the ScENO2 terminator from S. cerevisiae was used.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 925
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 855
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None