Coding
Part:BBa_K3394000
Designed by: Talia Shaler Group: iGEM20_BGU-Israel (2020-10-05)
Coding Sequence of Endo5a cellulase (for E. coli)
This part consists the coding region of Endo5A, an endo-1,4-beta-glucanases. Endo5a breaks down cellulose by making internal cuts in the cellulose chain. This enzyme is part of the complete cellulose hydrolysis reaction which involves several enzymes: endo-1,4-beta-glucanases, exo-1,4-beta-glucanases (or cellobiohydrolases) and beta-glucosidases.
It is a relatively small protein with an optimal temperature of 50 Celsius degrees and an optimal pH range of 6-7. The NCBI Genbank number for Endo5a is HQ657203.1 (DNA), AEB00655.1 (amino acid). This construct was synthesized by IDT for use in Escherichia coli.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 898
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 898
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 898
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 898
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 898
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |