Coding

Part:BBa_K3394000

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-05)
Revision as of 09:10, 5 October 2020 by Registry (Talk | contribs)

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Coding Sequence of Endo5a cellulase (for E. coli)

This part consists the coding region of Endo5A, an endo-1,4-beta-glucanases. Endo5a breaks down cellulose by making internal cuts in the cellulose chain. This enzyme is part of the complete cellulose hydrolysis reaction which involves several enzymes: endo-1,4-beta-glucanases, exo-1,4-beta-glucanases (or cellobiohydrolases) and beta-glucosidases.

It is a relatively small protein with an optimal temperature of 50 Celsius degrees and an optimal pH range of 6-7. The NCBI Genbank number for Endo5a is HQ657203.1 (DNA), AEB00655.1 (amino acid). This construct was synthesized by IDT for use in Escherichia coli.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 898
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 898
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 898
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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