Coding

Part:BBa_K3610008:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-08)
Revision as of 18:52, 3 October 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


EFR ectodomain - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 1012
    Illegal NheI site found at 1930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons that showed a codon frequency of less than 5% were changed into codons preferred by C. reinhardtii. If the preferred codon was interfering with the RFC standards, a codon with a lower codon frequency was chosen.

Source

Ordered from IDT

References