Part:BBa_K3610006:Design

EFR from A. thaliana - without signal sequence

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2683
  Illegal XbaI site found at 363
  Illegal XbaI site found at 2498
  Illegal SpeI site found at 1044
  Illegal PstI site found at 2337
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2683
  Illegal SpeI site found at 1044
  Illegal PstI site found at 2337
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2683
  Illegal BglII site found at 2348
  Illegal BglII site found at 2376
  Illegal BglII site found at 2919
  Illegal XhoI site found at 2372
  Illegal XhoI site found at 2962
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2683
  Illegal XbaI site found at 363
  Illegal XbaI site found at 2498
  Illegal SpeI site found at 1044
  Illegal PstI site found at 2337
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2683
  Illegal XbaI site found at 363
  Illegal XbaI site found at 2498
  Illegal SpeI site found at 1044
  Illegal PstI site found at 2337
  Illegal AgeI site found at 186
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The signal peptide was cleaved off out-of frame. For translation this part needs to be expressed with a signal sequence.

Source

Cyril Zipfel Lab at UZH, Switzerland

References

Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.

Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.

Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.

Pfeilmeier, Sebastian; George, Jeoffrey; Morel, Arry; Roy, Sonali; Smoker, Matthew; Stransfeld, Lena et al. (2019): Expression of the Arabidopsis thaliana immune receptor EFR in Medicago truncatula reduces infection by a root pathogenic bacterium, but not nitrogen-fixing rhizobial symbiosis. In: Plant biotechnology journal 17 (3), S. 569–579. DOI: 10.1111/pbi.12999.

Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.