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TIIS ter

Part:BBa_K3425020

Designed by: Núria Garriga Alonso, Björn Ancker Persson, Tereza Hubáčková, Dorottya Marko, Hui Yu   Group: iGEM20_UofUppsala   (2020-10-03)
Revision as of 11:28, 3 October 2020 by Registry (Talk | contribs)

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iGEM Type IIS standard terminator template

This part is the constitutive promoter BBa_J23105 adopted to the iGEM Type IIS standard (RFC1000). The BBa_J23105 sequence is flanked by the Type IIS prefix and suffix which allows cloning into the Level 0 backbone (pSB1C00). BBa_J23105 is part of the Anderson promoter family and its relative strength is 0.24. BBa_K3425017 can be ordered as two overlapping primers, see iGEM Type IIS guide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5
    Illegal SapI.rc site found at 39


[edit]
Categories
//collections/typeiis/uppsala
//collections/typeiis/uppsala/templates
Parameters
None