Coding
ClyR

Part:BBa_K3378000:Design

Designed by: Shengkun Tong   Group: iGEM20_HZAU-China   (2020-10-03)
Revision as of 07:14, 3 October 2020 by Registry (Talk | contribs)

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Chimeric lysin ClyR with activity against Streptococcus mutans.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 757
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 121
    Illegal AgeI site found at 205
    Illegal AgeI site found at 520
    Illegal AgeI site found at 703
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of this part has been optimized based on E. coli codon bias.


Source

The catalytic domain come from Streptococcus virus C1 and the cell-wall binding domain come from Streptococcus suis.

References