DNA

Part:BBa_K3570016

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-02)
Revision as of 00:15, 3 October 2020 by Antonmykhailiuk (Talk | contribs)


YPRCtau3 upstream homologous sequence

YPRCtau3 upstream homologous arm used together with YPRCtau3 downstream (BBa_K3570017). When YPRCtau3 upstream put to 5' of the biobrick together with YPRCtau3 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of type 4 long terminal repeat (Ty4 LTR) in chromosome XVI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 137
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None