DNA

Part:BBa_K3570016

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-02)
Revision as of 00:09, 3 October 2020 by Registry (Talk | contribs)

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YPRCtau3 upstream homologous sequence

YPRCdelta15 upstream homologous arm used together with YPRCdelta15 downstream (BBa_K3570015). When YPRCdelta15 upstream put to 5' of the biobrick together with YPRcΔ15 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within a region of long terminal repea (LTR) in chromosome XVI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 137
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 137
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 137
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None