Coding

Part:BBa_K3402008

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-01)
Revision as of 11:34, 2 October 2020 by ZZ 0991 (Talk | contribs)


NLS

Because of the high molecular weight of Cas9 nuclease which cannot freely shuttle through the nuclear pores, we have to add a nuclear localization sequence(NLS) to guide it through the nucleus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None