Coding

Part:BBa_K3349002

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-23)
Revision as of 22:14, 1 October 2020 by Kristiturton (Talk | contribs)


Pectin Lyase PelC

The PelC Pectin Lyase is originally from Paenabacillus amylolyticus . This particular pectin lyase is more specific to methylated pectin or homogalacturonan substrates rather than polygalacturonic acid [1]. This protein also works with both PelB(BBa_K3349001) and Pnl(BBa_K3349001) in this organism to effectively break down pectin. PelC has an optimal activity at 55°C and a pH of 10. The Km in in terms of 80% methylated substrates is 0.41 +/-0.06mg/ml. Calcium is a cofactor for this protein and is therefore necessary within the buffer conditions [1].

For purification purposes, this gene has a C-terminal hexa-histidine tag. For more information on our design and use please see our <a href="https://2020.igem.org/Team:Lethbridge_HS">webpage</a>.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 829
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 215


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Categories
Parameters
None