![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3610007:Design
Designed by: Jonas Sebastian Trottmann Group: iGEM20_UZurich (2020-09-08)
EFR Ectodomain without signal sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 82
Illegal NheI site found at 1012
Illegal NheI site found at 1930 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1598
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor. The signal sequence of the full length receptor was cleaved off out of frame.
Source
Cyril Zipfel Lab at UZH, Switzerland