Coding

Part:BBa_K3610007:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-08)
Revision as of 08:33, 1 October 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


EFR Ectodomain without signal sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 82
    Illegal NheI site found at 1012
    Illegal NheI site found at 1930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1598
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The juxtamembrane domain must be included in the sequence to ensure proper dimerization with the coreceptor

Source

PCR amplified from the full length receptor sequence.

References