Coding

Part:BBa_K3610005:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-24)
Revision as of 18:51, 30 September 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


EFR from A. thaliana


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2753
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2753
    Illegal BglII site found at 2418
    Illegal BglII site found at 2446
    Illegal BglII site found at 2989
    Illegal XhoI site found at 2442
    Illegal XhoI site found at 3032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
    Illegal AgeI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

Cyril Zipfel lab

References