Coding

Part:BBa_K3610004:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-24)
Revision as of 18:35, 30 September 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


BAK1 ectodomain from Arabidopsis thaliana - codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 778
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 708
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for C. reinhardtii. This sequence doesn't contain codons with a occurrence probability lower than 10%.

The juxtamembrane domain must be included in the sequence since the interaction with other plant receptors has shown to be weaker without this domain.

Source

Cyril Zipfel Lab at UZH, Switzerland Codon optimized with SnapGene

References