Coding

Part:BBa_K3610003:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-09-01)
Revision as of 18:26, 30 September 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


BAK1 Ectodomain from Arabidopsis thaliana


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 576
    Illegal PstI site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The juxtamembrane domain must be included in the sequence since the interaction with other plant receptors has shown to be weaker without this domain.

Source

PCR amplification from full length BAK1.

References