DNA

Part:BBa_K3570006

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-09-30)
Revision as of 09:46, 30 September 2020 by Antonmykhailiuk (Talk | contribs)


DPP1 upstream homologous sequence

DPP1 upstream homologous arm used together with DPP1 downstream (BBa_K3570007). When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do a homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 50
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None