Coding

Part:BBa_K3570008

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-09-30)
Revision as of 09:23, 30 September 2020 by Registry (Talk | contribs)

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HIS3 selection marker

The HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli.

When inserted to an integrative or replicative plasmid, HIS3 allows to counter-select the cells that acquired the wanted plasmid.

Sources 1- https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1005
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 896
    Illegal BglII site found at 956
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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