Coding

Part:BBa_K3349000

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-23)
Revision as of 16:31, 29 September 2020 by Kristiturton (Talk | contribs)


Pectin Lyase Pnl


This Pectin Lyase is from the organism Paenibacillus amylolyticus . Pnl cleaves at methylated sites of pectin or homogalacturonan substrates at high affinity [1]. Used in conjuction with Pectin lyases PelB and PelC, pectin can be effectively cleaved [1]. A hexa-histidine tag was added to the C-terminus of the coding sequence for nickel affinity purification.

For building thermostable variants of this Pnl enzyme we first did some homology modelling as the crystal structure has not been determined.


T--Lethbridge_HS--Pnl_modelling.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None