Coding

Part:BBa_K3349003:Design

Designed by: Kristi Turton   Group: iGEM20_Lethbridge_HS   (2020-09-23)
Revision as of 15:56, 23 September 2020 by Kristiturton (Talk | contribs) (→‎Design Notes)


Pectin Lyase Pnl-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for E. coli . The his tag was placed on the C-terminus. A TEV cut site was edded between the GFP and Pectase in case cleavage is needed after pruification.

Source

The GFP protein was taken from the uniprot database (P42212). The pectin lyase was also taken from the uniprot database and the sequence ID from NCBI is KAA8782864.1.

References